molecular study on the exon 2 region of the ovis bone morphology protein 15 (bmp-15) gene in iranian bluchi sheep breed by pcr-sscp technique
نویسندگان
چکیده
litter size is one of the most important economical traits in sheep breeding industry. in addition to minor genes, litter size is under the influence of major genes. bone morphogenetic protein 15 (bmp-15), a member of the transforming growth factor beta (tgf-ß) superfamily, which is specifically expressed in oocytes, plays a dramatic role in sheep prolificacy. reported mutations in this gene cause increased ovulation rate and infertility in a dosage-sensitive manner. six different point mutations have been indicated in the bmp-15 gene of sheep, each having a major effect on prolificacy. the aim of the current study was to investigate the lacaune (fecxl) mutation in the prolificacy of the iranian baluchi sheep breed. blood samples were collected in ethylenediaminetetraacetic acid(edta) coated tubes from jugular vein and genomic dna was extracted from whole blood samples. single nucleotide polymorphism of fecxl loci in bmp-15 gene were determined using polymerase chain reactionsingle-strand conformation polymorphism(pcr-sscp) technique. no evidence of mutation in fecxl was observed, all of which were monomorphic for exon 2 bmp-15 gene.
منابع مشابه
Molecular Study on the Exon 2 Region of the Ovis Bone Morphology Protein 15 (BMP-15) Gene in Iranian Bluchi Sheep Breed by PCR-SSCP Technique
Litter size is one of the most important economical traits in sheep breeding industry. In addition to minor genes, litter size is under the influence of major genes. Bone morphogenetic protein 15 (BMP-15), a member of the transforming growth factor beta (TGF-ß) superfamily, which is specifically expressed in oocytes, plays a dramatic role in sheep prolificacy. Reported mutations in this gene ca...
متن کاملAssociation of the Polymorphism Kiss1 Gene Exon 1 with Twin Traits in Sanjabi, Ghezel Breed Sheep by PCR–SSCP Technique
The Kiss1 gene is one of the known affective gene in fertility, which affects ovulation and multiplication. In this study, Blood samples were collected from 100 sheep of Sanjabi in Mehregan breeding station in Kermanshah province and 50 sheep Ghezel breeding station of Urmia University. After DNA extraction, the polymerase chain reaction (PCR) was used for specific primers amplification of 331 ...
متن کاملthe u.s. policy in central asia and its impact on the colored revolutions in the region (the case study of tulip revolution in kyrgyzstan)
چکیده ندارد.
15 صفحه اولa study on insurer solvency by panel data model: the case of iranian insurance market
the aim of this thesis is an approach for assessing insurer’s solvency for iranian insurance companies. we use of economic data with both time series and cross-sectional variation, thus by using the panel data model will survey the insurer solvency.
the effect of e-64 on the developmental competence of sheep cocs during in vitro maturation
in the present study, the effect of e-64 at different concentrations (0.5, 1, 5 and 10 µm) added to (1) the ivm medium on oocyte nuclear maturation and developmental competence of ovine oocytes, and (2) to the ivc medium on embryonic development of ovine embryos were investigated.
: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes
هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of applied animal scienceناشر: islamic azad university - rasht branch
ISSN 2251-628X
دوره 4
شماره 4 2015
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023